Effects of Low Temperature on Somatic Embryos Growth, Maturation and Planlet Regeneration of Citrus Mandarin var Batu 55 (Citrus reticulata Blanco.)
DOI:
https://doi.org/10.21776/ub.jels.2015.005.01.02Abstract
This study was aimed to determine the effect of incubation at 4°C temperature during multiplication stage of somatic embryos on growth, maturation and plantlets regeneration of citrus Mandarin var Batu 55. Globular somatic embryos were cultured on MT (Murashige & Tucker) medium and incubated at 4°C temperature for 0, 2, 4, 6, 8 weeks. Maturation of somatic embryos was induced by transfering globular somatic embryos on maturation medium (MT + 50 mgl-1 malt extract + 73 mM sorbitol + 73 mM galactose). Cotyledonary embryo was regenerated on MT medium + 50 mgl-1 malt extract + 30 gL-1 sucrose + 2 ppm GA3. The research showed that maturation of somatic embyos at 4°C temperature inhibited growth and maturation of somatic embyos. Fresh weight of somatic embryo incubated at 4°C for 2 weeks lower than fresh weight of embryo cultured without incubation at 4°C, and continuous decline in longer incubation period. Maturation percentage of embryos without incubation at 4°C temperature was 15%, but embryos incubated at 4°C temperature were lower than 9%. There was no effect of incubation at 4°C temperature during somatic embryos multiplication stage on plantlet regeneration percentage.
Keywords: Citrus reticulata, maturation, plantlet regeneration, somatic embryo
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).