Bone Marrow Cells Lymphocyte Activity of Pregnant Mice with Therapy of E. scaber and S. androgynus Post Infection Salmonella typhimurium

Muhammad Basyaruddin, Mochammad Sasmito Djati, Sri Rahayu

Abstract


Pregnancy can cause immune system changes. It is characterized by a decrease in the activity of immunocompetent cells. The use of antibiotics was intended to combat pathogenic microorganisms, but antibiotics have negative effects on pregnant women. The use of antibiotics can be replaced with extracts of Elephantopus scaber and Sauropus androgynus because both plants have chemical compounds that act as immunomodulators. This study was aimed to determine the activity of lymphocytes B220+, TER119+, and GR-1+ on bone marrow pregnant mice given the combination of extracts of Elephantopus scaber and Sauropus androgynus after infected with Salmonella typhimurium. This research uses seven treatment groups name: (K-) 0.05% NaCMC without bacterial infection; (K+) 0.05% NaCMC infected by bacteria; (P1) E. scaber 200 mg.kg-1BW infected by bacteria; (P2) E. scaber 150 mg.kg-1BW and S. androgynus 37.5 mg.kg-1BW infected by bacteria; (P3) E. scaber 100 mg.kg-1BW and S. androgynus 75 mg.kg-1BW infected by bacteria; (P4) E. scaber 50 mg.kg-1BW and S. androgynus 112.5 mg.kg-1BW infected by bacteria; and (P5) S. androgynus 150 mg.kg-1BW infected bacteria. The initial dose of E. scaber and S. androgynus was 50 mg.kg-1. Each treatment has three repetitions, surgery performed on day 12th and 18th. Lymphocyte cells isolated from bone marrow, the obtained results were analyzed by flowcytometry and statistical analysis using SPSS 16.0 one-way ANOVA, Tukey test and path. Based on the results from ANOVA tables, the formulations on mice that can restore their normal conditions with B220+ cells is E. scaber 150 mg.kg-1BW and S. androgynus 37.5 mg.kg-1BW, TER119+ cells is S. androgynus 150 mg.kg-1BW, while the GR-1+ cells affected by the surgery. Those three dose formulations can be used to obtain the optimum value which can increase the number of lymphocytes and not harmful to the developing fetus.

Keywords: Bone marrow, Elephantopus scaber, Lymphocyte, Pregnant mice, Sauropus androgynus

 


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DOI: http://dx.doi.org/10.21776/ub.jels.2015.005.01.09

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