Microsatellite Marker for Cross-Species Amplification: Study Case for Indonesian Sundaland Python (Serpentes: Pythonidae)

Authors

  • Andri Maulidi Departement of Biology, Faculty of Mathematics and Natural Science, Brawijaya University. Jl. Veteran, Malang 65145, East Java, Indonesia
  • Fatchiyah Fatchiyah Departement of Biology, Faculty of Mathematics and Natural Science, Brawijaya University. Jl. Veteran, Malang 65145, East Java, Indonesia
  • Amir Hamidy Laboratory of Herpetology, Museum Zoologicum Bogoriense, Research Center for Biology, Indonesian Institute of Sciences LIPI, Widyasatwa Loka Jl. Raya Jakarta Bogor km 46, Cibinong, West Java, Indonesia
  • Nia Kurniawan Departement of Biology, Faculty of Mathematics and Natural Science, Brawijaya University. Jl. Veteran, Malang 65145, East Java, Indonesia

DOI:

https://doi.org/10.21776/ub.jels.2018.008.01.10

Abstract

The python of Indonesian Sundaland has been traded for its distinct skin colour and patterns. The need for rapid method in cross-species amplification for Indonesian Sundaland python is useful to contribute in management of sustainability harvesting system. In this research, we screened 10 microsatellite primers which are previously used for Australian, New Guinean, Chinese and Burmese pythons and 7 potentially amplifiable primers for African and Asian reptiles. Python breitensteini showed a greater number of alleles (2-8 alleles) than Python bivittatus (1-3 alleles) and Python brongersmai (1-2 alleles). The observed and expected heterozygosity for all species were ranged from 0 to 1.00 and 0 to 0.79, respectively. According to the high cross-species amplification rates, 15 out of 17 primers were useful in assessing the genetic diversity and conservation genetic of Indonesian Sundaland python. Among the 15 primers, MS3 generated the highest number of allele for P. breitensteini (8 alleles), P. bivittatus (3 alleles), and P. brongersmai (2 alleles). We proposed MS3 locus as a suitable marker for Indonesian Sundaland python.

Keywords: microsatellite, Python, Sundaland.

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Published

2018-02-27

Issue

Vol. 8 No. 1 (2018)

Section

Articles

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Copyright (c) 2018 The Journal of Experimental Life Science

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