Growth Rate and Chemical Composition of Secondary Metabolite Extracellular Polysaccharide (EPS) in Microalga Porphyridium cruentum
AbstractPorphyridiumÂ cruentumÂ microalgae have great potential to be developed as a source of active ingredients in various fields of health.Â ItÂ excretes secondary metabolites in the form of extracellular polysaccharides (EPS), potentially as antibacterial, antihyperglycemic, and immunostimulant components. This study aims to obtain the best culture density of P.Â cruentum, the active component ofÂ P.Â cruentumÂ (EPS), and the chemical composition of EPS.Â The P.Â cruentumÂ was cultivated on 15%, 20%Â andÂ 25% culture stock, with an addition of silicate,Â FeÂ and vitamins in sterile seawater medium with salinity 35â€°, pH 8, temperature 25-27Â°C, with continuous aeration and 2500 lux continuous 24 hours, for 14 days of culture period. The results of this study indicate differences in growth rates and abundance of microalgae to each culture stock. The 15%, 20% and 25% culture stocks each showed stationary phase at days 10th, 12th and 14th, with EPS concentrations of 10 ppt, 12 ppt, and 15 ppt, respectively. Variation ofÂ P.Â cruentumÂ culture showed different EPS results, with a density of 25% capable of producing the highest EPS extract of 15,000 mg.L-1. The EPS is known to contain glucose and carboxylic acid compounds that can be utilized in the health and industrial fields.Keywords: culture, extracellular polysaccharides, Porphyridium cruentum.
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