Molecular Detection, Histopathology, and Scanning Electron Microscopy of Myxobolus koi Infecting Cyprinus carpio Koi

Rachmat Noer Soelistyoadi, Uun Yanuhar, Maftuch Maftuch

Abstract


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Myxobolus koi is a type of parasite that infects many freshwater fish through myxospores, resulting in a disease called myxobolosis. This research aimed is to investigate the clinical symptoms that occur in the gills of infected Koi carp (C. carpio). The utilized methods in this study are molecular detection, histopathology, and Scanning Electron Microscopy (SEM). Koi carp samples that are suspected to be infected with Myxobolus koi show several clinical signs, such as swollen and pale-colored gills and gill covers (opercula) that do not close completely, as well as white spots that appear on the edge of the lamella. The results of this study showed that through histological observations, there were changes characterized by hyperplasia of the primary lamella cartilage that envelops parasitic cysts and the encapsulation process that surrounds the cysts by gill cartilage in infected fish;SEM showed oval and elongated spores of a size of ± 12 µm (800x). TheDNA template from gill tissues in clinically infected and uninfected fish was examined byPCR testing with primers ERB 1 (Forward) and ERB 10 (Reverse); the results of electrophoresis in infected fish were detected at 2000 bp.

Keywords: Cyprinus carpio, Histopathology, Molecular Detection, Myxobolus koi, Parasite, SEM.


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DOI: http://dx.doi.org/10.21776/ub.jels.2019.009.03.01

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