The Use of Food Coloring Dyes in Bacterial Staining

Authors

  • Rio Risandiansyah Fakultas Kedokteran, Universitas Islam Malang.
  • Arniyati Arniyati Laboratorium Pusat Riset Kedokteran, Fakultas Kedokteran, Universitas Islam Malang
  • Nofie Irmalia Nurita Laboratorium Pusat Riset Kedokteran, Fakultas Kedokteran, Universitas Islam Malang
  • Natasya Hana Gionika

DOI:

https://doi.org/10.21776/ub.jels.2020.010.02.10

Abstract

Staining creates a contrast between the cells and its surrounding, and enables the microscopic characteristics of bacterial cells to be easily visible and distinguished. However, staining often relies on dyes which are expensive, not readily available, or toxic. In this study, the use of food coloring dyes to stain bacteria was explored. We stained Gram- positive bacteria (Bacillus sp. and Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) using several food coloring dyes of different colors, which were purchased locally. After slide fixation, the dye was flooded on the bacterial smear and air-dried for up to 30 minutes and observed by using microscope before and after washing with water. The results of this study show that prior to washing, most food coloring dyes were able to stain bacterial cells. However, after washing, only pink and purple food coloring dyes were retained, showing pink colored cells. We suspected that erythrosine was the agent responsible for this result, and was able to show similar characteristics with erythrosine alone. This study concludes that food coloring dyes containing erythrosine can be used to stain bacterial cells indiscriminately.

Keywords: Bacterial cell staining, erythrosine, food coloring dyes, resource-limited.

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Published

2021-01-25

Issue

Vol. 10 No. 2 (2020)

Section

Articles

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