Effect of Active Detergent Ingredients on Successful Fertilization and Embryo Development of Sea urchins Tripneustes gratilla (Linnaeus, 1758)
AbstractThe success of fertilization and development of sea urchin embryos Tripneustes gratilla can be used as a bioindicator of the water quality against the accumulation of pollutants. One of the contaminants that are often used is detergent with an active ingredient in the form of LAS. The purpose of this study was to analyze the effect of LAS on the success of fertilization and development of sea urchin embryos. This research was conducted in the Zoology Laboratory, Pattimura University, Ambon. Tripneustes gratilla were treated with exposure to the active ingredient LAS with a concentration of 0.0; 0.5; 1, and 2 mg.L-1. The parameter used to assess the success of fertilization is the formation of membrane fertilization. Parameters of embryo development are the division of 2, 4 , 8 , 16 , and 32 cells to form a morula, blastula, and hatching blastula. The results showed that the active ingredient of detergent LAS with a concentration of 0.5 mg.L-1 caused delays in the process of fertilization and embryo development. Meanwhile, the active ingredient LAS with concentrations of 1 and 2 mg.L-1 caused failure in the fertilization process and the embryonic development process of T. gratilla. Keywords: Detergent, embryo, fertilization, Tripneustes gratilla.
This work is licensed under a Creative Commons Attribution 4.0 International License.Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).