Isolation and Characterization of α-Amylase Enzyme on Brown Planthopper (Nilaparvata lugens Stal) On Rice
DOI:
https://doi.org/10.21776/ub.jels.2022.012.03.02Abstract
Brown leafhopper (Nilaparvata lugens Stal) is an important pest in Indonesia that causes heavy losses. The rice was damaged by N. Lugens sucking plant liquid in the form of carbohydrates in the form of starch. Environmentally friendly control methods are needed to reduce the increasingly high use of inorganic pesticides. Control of N. lugens with biotechnology can be done by inhibiting the metabolic cycle in these insects. This method utilizes the proteins available in rice seeds to stop the performance of enzyme α-amylase in the digestive system of N. lugens. α-amylase is an enzyme that plays a role in the process of starch degradation so that it becomes a simpler form both in microorganisms. The characterization of α-amylase enzymes in insects is an important first step to the determination of appropriate protein inhibitors so that they can be used to produce pest-resistant Genetically Modified Organisms crops. The research was conducted in the Agrotechnology laboratory, University of Jember. The research began with isolation and purification of α enzymes from brown stems and then continued with testing of α-amylase activity. Test parameters include the effect of temperature, pH, and substrate concentration on the activity of the α-amylase enzyme. The results showed that supernatant extracted from brown planthoppers produces a clear zone in the agar medium, which means the activity of enzymes α-amylase in the hydrolysis of starch. The pH value of 6 provides the most optimum conditions for the activity of α enzymes. The α-amylase enzyme is able to work optimally in the temperature range of 30°C - 45°C, and experiences a decrease in activity when the temperature reaches 50°C. The α-amylase enzyme shows the ability to hydrolyze the amylase substrate to a concentration of 0.8 μg.μL-1.
Keywords: α-amylase, Nilaparvata lugens Stal, Rice.
Downloads
Published
Issue
Section
License

This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).