The Effect of Alpinia Purpurata K. Schum Rhizome Extract Supplementation in Tris-Egg Yolk Extender on the Quality of Cryopreserved Bali Bull Sperm

Authors

  • Firlina Laila Putri Universitas Brawijaya, Malang, Indonesia
  • Sri Rahayu Universitas Brawijaya, Malang, Indonesia
  • Agung Pramana Warih Marhendra Universitas Brawijaya, Malang, Indonesia

DOI:

https://doi.org/10.21776/ub.jels.2023.013.02.03

Abstract

This study aimed to analyze the effect of Alpinia purpurata K. Schum rhizome extract (APRE) supplementation into tris-egg yolk extender on the sperm quality of Bali bull after freezing. This study used experimental method with a completely randomized design (CRD), consisting of five treatments, denoted as P0 (semen diluted using 100% Tris-Egg Yolk (TEY)), P1 (semen diluted using 98% TEY and 2% APRE), P2 (semen diluted using 96% TEY extender + 4% APRE), P3 (semen diluted using 94% TEY + 6% APRE), and P4 (semen diluted using 92% TEY + 8% APRE). Semen quality was assessed both before and after freezing, using several indicators such as motility, viability, and abnormality of sperm. The antioxidant activity of RGE was determined using the DPPH (1,1-diphenils-2-picrylhydrazils) technique. The data were analyzed using ANOVA with further analysis using post hoc tests in the SPSS 25 program. The results showed that APRE has strong antioxidant activity, with an IC50 value 38.004 μg.mL-1. Meanwhile, this research has shown that the addition of 6% RGE in the tris-Tris-egg yolk extender was able to maintain sperm quality before and after freezing.

Keywords: Alpinia purpurata K. Schum, sperm quality, tris-egg yolk.

Author Biographies

Firlina Laila Putri, Universitas Brawijaya, Malang, Indonesia

Master Program of Biology, Faculty of Mathematics and Natural Sciences

Sri Rahayu, Universitas Brawijaya, Malang, Indonesia

Department of Biology, Faculty of Mathematics and Natural Sciences

Agung Pramana Warih Marhendra, Universitas Brawijaya, Malang, Indonesia

Department of Biology, Faculty of Mathematics and Natural Sciences

Downloads

Published

2023-07-11

Issue

Vol. 13 No. 2 (2023)

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

    1. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

  1. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).