Isolation of Nitrogen-Fixing Bacteria from Rhizosphere of Sugarcane Plantation as Candidates for Biofertilizer

Authors

  • Altayib Hasan Mohammed Ahmed Bin Yahmed 1) Universitas Brawijaya, Malang, Indonesia ; 2) Fezzan University, Libya
  • Irfan Mustafa Universitas Brawijaya, Malang, Indonesia
  • Farida Rahayu National Research and Innovation Agency (BRIN), Indonesia

DOI:

https://doi.org/10.21776/ub.jels.2025.015.01.04

Abstract

Exploration of nitrogen-fixing bacteria as a sustainable alternative to conventional fertilizers is important in agriculture. The study aims to isolate and characterize the potential nitrogen-fixing bacteria (NFB) from soils of several sugarcane fields in East Java. Specifically, the bacteria were isolated from sugarcane fields in Blitar, Ngawi, and Glugur Malang. Soil samples were collected, and NFB were isolated using an Nfb medium. Next, the ability of these isolates to fix nitrogen in a liquid medium was evaluated by measuring the ammonium released after different incubation periods (24, 72, and 120 hours). The study obtained five potential isolates that grew well on the agar medium and changed the medium’s pH. Significant growth and pH increase at the liquid medium were observed across the isolates over time. This rise in pH was not always attributed to the ammonia production by those NFB. Only two isolates released ammonia at detectable concentrations as much as 1.13 mg.L-1 and 1.40 mg.L-1, respectively. Hence, isolates C and D were selected as potential NFB for application in replacing synthetic nitrogen fertilizer.

Keywords: biofertilizer, environmental sustainability, nitrogen-fixing bacteria, rhizosphere.

Author Biographies

Altayib Hasan Mohammed Ahmed Bin Yahmed, 1) Universitas Brawijaya, Malang, Indonesia ; 2) Fezzan University, Libya

1Master Program of Biology, Department of Biology, Faculty of Mathematics and Natural Sciences
2Faculty of Medical Technology

Irfan Mustafa, Universitas Brawijaya, Malang, Indonesia

Department of Biology, Faculty of Mathematics and Natural Sciences

Farida Rahayu, National Research and Innovation Agency (BRIN), Indonesia

Research Center for Applied Microbiology

Downloads

Published

2025-02-28

Issue

Vol. 15 No. 1 (2025)

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

    1. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

  1. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).