The The Effect of Gamma Irradiation on In Vitro Explant Growth, Shoot Multiplication, and Plantlet Regeneration Explants Bulbil of Porang (Amorphophallus muelleri Blume)
DOI:
https://doi.org/10.21776/ub.jels.2025.015.01.06Abstract
Porang (Amorphophallus muelleri Blume) is a tuberous plant that contains glucomannan as an alternative in reducing obesity. It is widely traded, but it is cultivated through conventional methods and takes a long time. In vitro mutagenesis technique with gamma irradiation is expected to increase the production of superior porang. This study aims to determine the effect of gamma irradiation on explant growth, shoot multiplication, and plantlet regeneration on in vitro culture. The doses of gamma irradiation, i.e. 0, 2, 4, 6, 8, and 10 gamma rays (Gy). Explants bulbil were precultured for 14 days on Murashige and Skoog (MS) medium fortified with 6-benzyl amino purine (BAP) 3 mg.L-1 before irradiation. The irradiated explants were then cultured on media containing MS supplemented with α-naphthalene acetic acid (NAA) 0.1 mg.L-1 and BAP 3 mg.L-1 for 4 and 8 weeks of culture. Plantlet regeneration media was MS supplemented with NAA 0.1 mg.L-1 for 4 weeks of culture. The results showed that a dose of 10 Gy gamma irradiation was able to inhibit explant growth (0.06 g), shoot multiplication (0%), and plantlet height (0.38 cm), while gamma irradiation at a dose of 6 Gy increased explant growth (0.16 g), shoot multiplication (40%), and plantlet height (5.60 cm). The lethal dose 50 (LD50) of gamma irradiation for explant survival rate is 8.79 Gy.
Keywords: Amorphophallus muelleri, explant growth, gamma irradiation, in vitro, plantlet regeneration, shoot multiplication
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
