Tapak Liman (Elephantopus scaber L.) Leaves Ethanol Extract Improves the Production of IL-6 and IL-17 Cytokines in Mice with Bleomycin-induced Pulmonary Fibrosis

Authors

  • Sarah Nahdah Nabilah Universitas Brawijaya, Malang, Indonesia
  • Yuyun Ika Christina Universitas Brawijaya, Malang, Indonesia
  • Dinia Rizqi Dwijayanti Universitas Brawijaya, Malang, Indonesia
  • Muhaimin Rifa’i Universitas Brawijaya, Malang, Indonesia
  • Muhammad Sasmito Djati Universitas Brawijaya, Malang, Indonesia

DOI:

https://doi.org/10.21776/ub.jels.2024.014.01.02

Abstract

IL-6 and IL-17 are pro-inflammatory and pro-fibrotic cytokines that increase pulmonary fibrosis due to lung alveolar epithelial cell damage. Tapak liman leaves (Elephantopus scaber L.) have anti-inflammatory and anti-asthmatic properties. This study aimed to investigate the effects of the Elephantopus scaber L. ethanol extract (ESEE) on IL-6 and IL-17 produced by CD4+ and CD8+ in the bleomycin-induced pulmonary fibrosis mice model. Fifty-six male BALB/c mice will be divided into seven groups consisting of healthy mice (N), vehicle mice (V), pulmonary fibrosis (PF), Dexamethasone (DEX) as a drug control, and three doses of ESEE (0.0504, 0.1008, and 0.2016 mg.kg-1 BW). ESEE will be administered orally, followed by intraperitoneal bleomycin injection for 14 days. Mice are then dissected on days 7 and 14, and the spleen will be isolated for analysis of the expression of IL-6 and IL-17. The results showed that ESEE effectively reduced levels of IL-6 and IL-17 cytokines produced by CD4+ and CD8+ T cells, and doses three of ESEE (0.2016 mg.kg-1 BW)  (0.2016 mg.kg-1 BW) showed the most effective reduction activity than the Dexamethasone group. The treatment was proven to reduce the expression of IL-6 and IL-17 in mice with a model of pulmonary fibrosis.

Keywords: bleomycin, Elephantopus scaber L., IL-6, IL-17, pulmonary fibrosis.

Author Biographies

Sarah Nahdah Nabilah, Universitas Brawijaya, Malang, Indonesia

Bachelor Program of Biology, Department of Biology, Faculty of Mathematics and Natural Sciences

Yuyun Ika Christina, Universitas Brawijaya, Malang, Indonesia

Department of Biology, Faculty of Mathematics and Natural Sciences

Dinia Rizqi Dwijayanti, Universitas Brawijaya, Malang, Indonesia

Department of Biology, Faculty of Mathematics and Natural Sciences

Muhaimin Rifa’i, Universitas Brawijaya, Malang, Indonesia

Department of Biology, Faculty of Mathematics and Natural Sciences

Muhammad Sasmito Djati, Universitas Brawijaya, Malang, Indonesia

Department of Biology, Faculty of Mathematics and Natural Sciences

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Published

2024-02-29

Issue

Vol. 14 No. 1 (2024)

Section

Articles

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