Phytochemical Analysis of Purple Sweet Potatoes (Ipomoea batatas) Roots Extract From Lawang and Kawi Mountain Cultivar, East Java, Indonesia

Authors

DOI:

https://doi.org/10.21776/ub.jels.2022.012.01.03

Keywords:

purple sweet potatoes, anthocyanin, antioxidant, geographical

Abstract

Indonesia has wide cultivation of purple sweet potatoes (PSP) commodities, particularly in East Java province. However, the difference of phytochemical profiles in PSP among geographical regions has not been fully explored. This study aimed to analyze the phytochemicals, anthocyanin, and antioxidant activity profiles from two different cultivars of PSP from Lawang and Kawi Mountain region, East Java, Indonesia. The acidified methanol extract was identified for a phytochemical compound using standard methods. Antioxidant activity was analyzed using a ferric reducing assay. Anthocyanins were screened using ultraviolet-visible spectroscopy and total calculation. Both extracts have positive values in their alkaloid, phenolic, flavonoid, glycoside, and tannin content. Antioxidant activity was high with IC50 value 2.5 and 2.3 µg. mL-1 for Lawang and Kawi Mountain, respectively. Each cultivar has a similar peak at 521 nm at pH 1 and 530 nm at pH 4.5. Total anthocyanin calculation was showed that Lawang has higher anthocyanin content than Kawi Mountain cultivar. We concluded that PSP from Lawang has better anthocyanin content than the Kawi Mountain cultivar. We proposed that PSP from the Lawang cultivar has the potential to be explored in further research and health-related product development. Keywords: anthocyanin, antioxidant, geographical,  purple sweet potatoes.

Author Biographies

Fajar Mustika Alam, Universitas Brawijaya, Indonesia

Bachelor Program of Biology Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Malang, Indonesia

Nia Kurnianingsih, Universitas Brawijaya

Department of Physiology, Faculty of Medicine, UniversitasBrawijaya, Malang, Indonesia

Fatchiyah Fatchiyah, Universitas Brawijaya, Malang, Indonesia

       Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Malang, IndonesiaResearch Center of Smart Molecules of Natural Genetic Resources, Universitas Brawijaya,Malang, Indonesia

References

Chandrasekara, A and Kumar, TJ. 2016.Roots and tuber crops as functional foods: A review on phytochemical constituents and their potential health benefits, Int. J. Food Sci. 2016 (Article ID 3631647):1-15. http://dx.doi.org/10.1155/2016/3631647

Suryani,R.2016.Outlook Komoditas Pertanian Sub Sektor Tanaman Pangan. Pusat Data dan Sistem Informasi Pertanian Kementerian Pertanian. Indonesia, pp.28.

Dewi, R., Utomo, S.D., Kamal, M., Timotiwu, PB and Nurdjanah,S.2019.Genetic and phenotypic diversity, heritability, and correlation between the quantitative characters on 30 sweet potato germplasms in Lampung, Indonesia. Biodiversitas. 20(2):380–386.

Ariadi, BY.2006. Comparison Among Various Sweet Potato Varieties Focused From Farm Income and Marketing At Malang Regency. Jurnal Pembangunan Pedesaaan. 6(2):103-113.

Kurnianingsih, N.,Ratnawati, R., Nazwar, TA., Ali, M and Fatchiyah,F.2021.Purple Sweet Potatoes from East Java of Indonesia Revealed the Macronutrient, Anthocyanin Compound and Antidepressant Activity Candidate. Med. Arch.75(2):94-100. doi: 10.5455/medarh.2021.75.94-100.

Li, A., Xiao, R., He, S., An, X., He, Y., Wang, C. et al.2019.Research advances of purple sweet potato anthocyanins: Extraction, identification, stability, bioactivity, application, and biotransformation.Molecules. 24(3816):1-21.doi:10.3390/molecules24213816.

Mukherjee, PK., Harwansh, RK., Bahadur, S., Duraipandiyan, V and Al-Dhabi, NA.2017. Factors to Consider in Development of Nutraceutical and Dietary Supplements in Current Trends in Pharmacognosy Research. Elsevier Inc.USA. pp.663-661.doi.org/10.1016/B978-0-12-802104-0.00034-2.

Prabawati, RK., Ratnawati, R., Rahayu, M and Prakosa, AG.2019.Effect Anthocyanin of Purple Potato Gunung Kawi on MDA Levels, Expression of Caspase-3, and Spatial Memory Function on Diabetic Wistar Rats.Malang Neurol. Journal.5(1):34–41.

Suhardi, C., Ratnawati, R and Khotimah, H.2016.Pengaruh Pemberian Antosianin Dari Ipomoea batatas L Varietas Ungu Kultivar Gunung Kawi Dalam Meningkatkan Kadar Superoxide Dismutase Pada Tikus (Rattus novergicus) Dengan Diet Aterogenik.Majalah Kesehatan.3(4):166–173.

Kurnianingsih, N., Ratnawati,R., Fatchiyah, F., Barlianto, W., Ali, M., Safitri, A and Suyanto, E.2019.The Difference of Amino Acid Profiling From Two Morphological Purple The Difference of Amino Acid Profiling From Two Morphological Purple Sweet Potatoes From Kawi Mountain Cultivars , East Java , Indonesia. Journal of Phys. Conf. Ser.1374(012017):3–8.

Yaningsih, H., Admadi, B and Mulyani,S.2016.Studi Karakteristik Gizi Ubi Jalar Ungu ( Ipomoea batatas var Gunung Kawi ) Pada Beberapa Umur Panen. Jurnal Rekayasa dan Manajemen Agroindustri.1(1):21–30.

Ramakrishna, A and Ravishankar, GA.2011.Influence of abiotic stress signals on secondary metabolites in plants. Plant Signal. Behav.6(11):1720–1731. doi: 10.4161/psb.6.11.17613.

Fatchiyah, F., Tirtosari, DR., Safitri, A and Cairns, JRK.2020.Phytochemical Compound and Nutritional Value in Black Rice from Java Island , Indonesia. Sys Rev Pharm.11(7):414–421.

Amelia, F., Afnani, GN., Musfiroh, A., Fikriyani, AN and Ucche, S.2013.Extraction and Stability Test of Anthocyanin from Buni Fruits (Antidesma Bunius L) as an Alternative Natural and Safe Food Colorants. J. Food Pharm Sci.1(2): 49–53.

Chen, CC., Lin, C., Chen, MH and Chiang, PY.2019.Stability and quality of anthocyanin in purple sweet potato extracts. Foods.8(9):1–13.

Ahmed, D., Khan, MM and Saeed, R.2015.Comparative analysis of phenolics, flavonoids, and antioxidant and antibacterial potential of methanolic, hexanic and aqueous extracts from Adiantum caudatum leaves. Antioxidants.4(2):394–409.

Nugroho, DW and Kamila, M.2020.Effect of Mechanical Milling on the Total Phenolic Content and Antioxidant Activity of Garcinia mangostana Pericarp.Makara J. Sci. 24(2): 2020.

Wang, H., Fan, W., Li, H., Yang, J., Huang, J and Zhang, P.2013.Functional characterization of dihydroflavonol-4-reductase in anthocyanin biosynthesis of purple sweet potato underlies the direct evidence of anthocyanins function against abiotic stresses.PLoS One.8(11):1–14.

Brown, CR.2005.Antioxidants in potato.Am. J. Potato Res.82(2):163–172.

Ghasemzadeh, A., Talei, D., Jaafar, HZE., Juraimi, AS., Mohamed, MTM., Puteh, A and Halim, MRA.2016.

Plant-growth regulators alter phytochemical constituents and pharmaceutical quality in Sweet potato (Ipomoea batatas L.). BMC Complement. Altern. Med.16(1):1–13.

Sendangratri, Handayani, R and Elya, B.2018.Inhibitory effects of different varieties of sweet potato (Ipomoea batatas L.) tubers extracts on lipoxygenase activity.Pharmacogn. J.11(6):1195–1198.

Dewi, L.T., Adnyana, MO., Mahdi, C., Prasetyawan, S., Srihardyastutie, A and Aulanni’am, A.2018.Study of Antocyanins Activity from Purple Sweet Potato for Reducing Apoptotic Cells Expression of The Cerebellum On Ischemic Stroke Rats. J. Pure Appl. Chem. Res.7(2):94–99.

Setyati, D., Sulistiyowati, H., Erizcy, MP and Ratnasari, T.2020.The Flavonoid and Alkaloid Content of Cyclosorus Parasiticus (Linn.) Farwell Ferns At the Plantation Areas of Jember Regency.BIOLINK (Jurnal Biol. Lingkung. Ind. Kesehatan).7(1):23–37.

Saha,S., Singh, J., Paul, A., Sarkar, R., Khan, Z and Banerjee, K.2021.Anthocyanin profiling using UV-vis spectroscopy and liquid chromatography mass spectrometry. J. AOAC Int.103(1):23–39.

Khusnia, A.2017.Keanekaragaman serangga tanah di perkebunan teh PTPN XII Wonosari Lawang.Undergraduate Thesis.UIN Maulana Malik Ibrahim, Malang.

Putra, MJNFI, Soemarno, S and Suntari,S.2016.Humification degree and its relationship with some soil physical characteristics on robusta coffee (Coffea canephora) plantation. J. Degrad. Min. Lands Manag.3(4): 649–658.

Thakur, M and Sohal, BS.2013.Role of Elicitors in Inducing Resistance in Plants against Pathogen Infection: A Review. ISRN Biochem. 2013(Article ID 762412):1-10.doi:10.1155/2013/762412

Vuolo, MM., Lima, S and Junior, MRM.2018.Phenolic Compounds: Structure, Classification, and Antioxidant Power in Bioactive Compounds. Elsevier Inc.USA.pp.33-50.doi:10.1016/B978-0-12-814774-0.00002-5.

Wang, SY and Zheng, W.2001.Effect of plant growth temperature on antioxidant capacity in strawberry.J. Agric. Food Chem.49(10):4977–4982.

Thoma, F., Somborn-Schulz A., Schlehuber, D., Keuter, V and Deerberg, G.2020.Effects of Light on Secondary Metabolites in Selected Leafy Greens: A Review.Front. Plant Sci.11(497):1–15. doi: 10.3389/fpls.2020.00497

Pascual, MB., El-Azaz, J., de la Torre, FN., Cañas, RA., Avila, C and Cánovas, FM.2016.Biosynthesis and metabolic fate of phenylalanine in conifers.Front. Plant Sci.7(1030):1-13.doi: 10.3389/fpls.2016.01030.

Winkel-shirley, B.2001.Flavonoid Biosynthesis. A Colorful Model for Genetics, Biochemistry, Cell Biology, and Biotechnology. Plant Physiol. 126:485–493.

Wegener, CB and Jansen, G.2013.Antioxidants in different potato genotypes: Effect of drought and wounding stress.Agric.3(1):pp. 131–146.

John, KS., George, J., Beegum, SUS and Shivay, YS.2016.Soil fertility and nutrient management in tropical tuber crops - An overview.Indian J. Agron.61(3):263–273.

Kunhikrishnan, A., Thangarajan, R., Bolan, NS., Xu, Y., Mandal, S., Gleeson, DB, et al.2016.Functional Relationships of Soil Acidification, Liming, and Greenhouse Gas Flux in Adv. Agron. vol. 139. Elsevier.Inc.USA.pp. 1–71.

Jezek, M., Zörb, C., Merkt, N and Geilfus,CM.2018.Anthocyanin Management in Fruits by Fertilization. J. Agric. Food Chem.66:753–764.

Hhirley, BW., Kubasek, WL., Storz, G., Bruggemann, E., Koornneef, M., Ausubel, FM and Goodman, HM.1995.Analysis of Arabdidopsis mutants deficient in flavonoid biosynthesis.Plant J.8(5):659–671.

Montilla, EC., Hillebrand, S and Winterhalter, P.2011.Anthocyanins in purple sweet potato (Ipomoea batatas L .) Varieties.Fruit, Veg. Cereal Biotechnol.5(Special Issue 2):19–24.

Kim, YH., Lee, YS., Kim,MK., Kim, HS., Kim, H and Koh, K.2009.Identification and quantification of anthocyanin pigments in colored rice.Nutr. Res. Pract.2(1):46.

Sharma, V and Janmeda, P.2017.Extraction, isolation and identification of flavonoid from Euphorbia neriifolia leaves. Arab. J. Chem.10(4) :509–514.

Downloads

Additional Files

Published

2022-03-02

Issue

Section

Articles